Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Background: Fasciolosis is a global parasitic disease caused by liver trematodes such as Fasciola hepatica, affecting various mammals, including sheep, goats, cattle, pigs, and humans worldwide. Cathepsin L (Cat L) found in the life stages of F. gigantica associated with pathology fluke. Given detrimental impact fasciolosis on animal health production, along reports drug resistance concerns regarding residues products environment, there has been an increasing emphasis developing potent epitope-based vaccine to combat this infection. Objectives: The present study aimed at bioinformatically analyzing characteristics Cat newly encysted juvenile (FgNEJ) identify potential epitopes for constructing effective against Methods: FgNEJ was analyzed using several reliable bioinformatics tools. Results: Analysis second structure amino acid sequence demonstrated that alpha-helix, random coil, extended strand ratios were 77 (23.62%), 164 (50.31%), 85 (26.07%), respectively. B-cell, T-cell, MHC predicted suggested ideal target design. Conclusions: current introduces significant information FgNEJ, well some promising can be used strong candidates studies involved fasciolosis.